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人抗核周因子抗體酶免試劑盒,(APF)ELISA檢測試劑盒,英文名:APF ELISA Kit,英文縮寫:APF ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗核周因子抗體ELISA檢測試劑盒,APF 檢測試劑盒,APF 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人抗核周因子抗體酶免試劑盒,(APF)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重
蛋白激酶抑制因子γ(PKIγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase Inhibitor Gamma (PKIg)48T/96TY08685Mus musculus (Mouse)
血管內(nèi)皮生長因子A(VEGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)48T/96TY08686Oryctolagus cuniculus (Rabbit)
血管性血友病因子(vWF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Von Willebrand Factor (vWF)48T/96TY08687Oryctolagus cuniculus (Rabbit)
血管緊張素Ⅰ(AngⅠ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiotensin I (AngI)48T/96TY08688Homo sapiens (Human)
血管緊張素Ⅰ(AngⅠ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiotensin I (AngI)48T/96TY08689Rattus norvegicus (Rat)
血管緊張素Ⅰ(AngⅠ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiotensin I (AngI)48T/96TY08690Mus musculus (Mouse)
細(xì)胞色素C(CYCS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytochrome C, Somatic (CYCS)48T/96TY08691Homo sapiens (Human)
細(xì)胞色素C(CYCS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytochrome C, Somatic (CYCS)48T/96TY08692Rattus norvegicus (Rat)
細(xì)胞色素C(CYCS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytochrome C, Somatic (CYCS)48T/96TY08693Mus musculus (Mouse)
白三烯C4(LTC4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukotriene C4 (LTC4)48T/96TY08694General
胰淀素(IAPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Islet Amyloid Polypeptide (IAPP)48T/96TY08695Homo sapiens (Human)
胰淀素(IAPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Islet Amyloid Polypeptide (IAPP)48T/96TY08696Rattus norvegicus (Rat)
胰淀素(IAPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Islet Amyloid Polypeptide (IAPP)48T/96TY08697Mus musculus (Mouse)
低氧誘導(dǎo)因子1α(HIF1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)48T/96TY08698Homo sapiens (Human)
低氧誘導(dǎo)因子1α(HIF1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)48T/96TY08699Rattus norvegicus (Rat)
低氧誘導(dǎo)因子1α(HIF1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)48T/96TY08700Mus musculus (Mouse)
B-淋巴細(xì)胞激活抗原B7-2(LAB7-2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for B-Lymphocyte Activation Antigen B7-2 (LAB7-2)48T/96TY08701Homo sapiens (Human)
B-淋巴細(xì)胞激活抗原B7-2(LAB7-2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for B-Lymphocyte Activation Antigen B7-2 (LAB7-2)48T/96TY08702Rattus norvegicus (Rat)
B-淋巴細(xì)胞激活抗原B7-2(LAB7-2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for B-Lymphocyte Activation Antigen B7-2 (LAB7-2)48T/96TY08703Mus musculus (Mouse)
白介素6(IL6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 6 (IL6)48T/96TY08704Chicken (Gallus)
干擾素γ(IFNγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Gamma (IFNg)48T/96TY08705Chicken (Gallus)
90kDa熱休克蛋白β1(HSP90β1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1)48T/96TY08706Homo sapiens (Human)
90kDa熱休克蛋白β1(HSP90β1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1)48T/96TY08707Rattus norvegicus (Rat)
90kDa熱休克蛋白β1(HSP90β1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1)48T/96TY08708Mus musculus (Mouse)
超氧化物歧化酶(SOD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Superoxide Dismutases (SOD)48T/96TY08709Homo sapiens (Human)
超氧化物歧化酶1(SOD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Superoxide Dismutase 1, Soluble (SOD1)48T/96TY08710Rattus norvegicus (Rat)
超氧化物歧化酶1(SOD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Superoxide Dismutase 1, Soluble (SOD1)48T/96TY08711Mus musculus (Mouse)
丙二醛(MDA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Malondialdehyde (MDA)48T/96TY08712General
60kD熱休克蛋白(HSPD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 60kD Protein 1, Chaperonin (HSPD1)48T/96TY08713Homo sapiens (Human)
60kD熱休克蛋白(HSPD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 60kD Protein 1, Chaperonin (HSPD1)48T/96TY08714Rattus norvegicus (Rat)
60kD熱休克蛋白(HSPD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 60kD Protein 1, Chaperonin (HSPD1)48T/96TY08715Mus musculus (Mouse)
血管緊張素原(AGT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiotensinogen (AGT)48T/96TY08716Homo sapiens (Human)
血管緊張素原(AGT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiotensinogen (AGT)48T/96TY08717Rattus norvegicus (Rat)
血管緊張素原(AGT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiotensinogen (AGT)48T/96TY08718Mus musculus (Mouse)
脂質(zhì)轉(zhuǎn)運蛋白(CETP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholesteryl Ester Transfer Protein (CETP)48T/96TY08719Homo sapiens (Human)
神經(jīng)型一氧化氮合酶()檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase 1, Neuronal ()48T/96TY08720Homo sapiens (Human)
神經(jīng)型一氧化氮合酶()檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase 1, Neuronal ()48T/96TY08721Mus musculus (Mouse)
神經(jīng)型一氧化氮合酶()檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase 1, Neuronal ()48T/96TY08722Rattus norvegicus (Rat)
內(nèi)皮型一氧化氮合酶(NOS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3)48T/96TY08723Sus scrofa; Porcine (Pig)
內(nèi)皮型一氧化氮合酶(NOS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3)48T/96TY08724Cavia (Guinea pig )
C反應(yīng)蛋白(CRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C Reactive Protein (CRP)48T/96TY08725Bos taurus; Bovine (Cattle)
α1-酸性糖蛋白(α1AGP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)48T/96TY08726Homo sapiens (Human)
α1-酸性糖蛋白(α1AGP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)48T/96TY08727Bos taurus; Bovine (Cattle)
α1-酸性糖蛋白(α1AGP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)48T/96TY08728Rattus norvegicus (Rat)
α1-酸性糖蛋白(α1AGP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)48T/96TY08729Mus musculus (Mouse)
銅藍(lán)蛋白(CP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ceruloplasmin (CP)48T/96TY08730Rattus norvegicus (Rat)
結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Haptoglobin (Hpt)48T/96TY08731Homo sapiens (Human)
結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Haptoglobin (Hpt)48T/96TY08732Bos taurus; Bovine (Cattle)
結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Haptoglobin (Hpt)48T/96T Y08733Mus musculus (Mouse)
結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Haptoglobin (Hpt)48T/96TY08734Rattus norvegicus (Rat)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。