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人抗單核細(xì)胞抗體酶免試劑盒,(AMA)ELISA檢測試劑盒,英文名:AMA ELISA Kit,英文縮寫:AMA ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗單核細(xì)胞抗體ELISA檢測試劑盒,AMA 檢測試劑盒,AMA 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人抗單核細(xì)胞抗體酶免試劑盒,(AMA)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重
GATA結(jié)合蛋白2(GATA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for GATA Binding Protein 2 (GATA2)48T/96TY08885Mus musculus (Mouse)
蛋白激酶抑制因子β(PKIβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase Inhibitor Beta (PKIb)48T/96TY08886Rattus norvegicus (Rat)
蛋白激酶抑制因子β(PKIβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase Inhibitor Beta (PKIb)48T/96TY08887Mus musculus (Mouse)
解整合素金屬蛋白酶8(ADAM8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 8 (ADAM8)48T/96TY08888Homo sapiens (Human)
解整合素金屬蛋白酶8(ADAM8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 8 (ADAM8)48T/96TY08889Rattus norvegicus (Rat)
解整合素金屬蛋白酶8(ADAM8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 8 (ADAM8)48T/96TY08890Mus musculus (Mouse)
淀粉酶β(Amyβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Amylase Beta (Amyb)48T/96TY08891Homo sapiens (Human)
胰淀粉酶α2(AMY2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Amylase Alpha 2, Pancreatic (AMY2)48T/96TY08892Mus musculus (Mouse)
碳酸酐酶Ⅱ(CA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carbonic Anhydrase II (CA2)48T/96TY08893Homo sapiens (Human)
碳酸酐酶Ⅱ(CA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carbonic Anhydrase II (CA2)48T/96TY08894Rattus norvegicus (Rat)
碳酸酐酶Ⅱ(CA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carbonic Anhydrase II (CA2)48T/96TY08895Mus musculus (Mouse)
乳鐵傳遞蛋白(LTF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactoferrin (LTF)48T/96TY08896Homo sapiens (Human)
乳鐵傳遞蛋白(LTF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactoferrin (LTF)48T/96TY08897Rattus norvegicus (Rat)
乳鐵傳遞蛋白(LTF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactoferrin (LTF)48T/96TY08898Mus musculus (Mouse)
羥脯氨酸(Hyp)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hydroxyproline (Hyp)48T/96TY08899General
肝癌源性生長因子(HDGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hepatoma Derived Growth Factor (HDGF)48T/96TY08900Homo sapiens (Human)
肝癌源性生長因子(HDGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hepatoma Derived Growth Factor (HDGF)48T/96TY08901Rattus norvegicus (Rat)
肝癌源性生長因子(HDGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hepatoma Derived Growth Factor (HDGF)48T/96TY08902Mus musculus (Mouse)
中性粒細(xì)胞彈性蛋白酶(ELA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Elastase 2, Neutrophil (ELA2)48T/96TY08903Homo sapiens (Human)
胱天蛋白酶9(CASP9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Caspase 9 (CASP9)48T/96TY08904Mus musculus (Mouse)
BH3相互作用域死亡激動劑(Bid)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for BH3 Interacting Domain Death Agonist (Bid)48T/96TY08905Homo sapiens (Human)
BH3相互作用域死亡激動劑(Bid)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for BH3 Interacting Domain Death Agonist (Bid)48T/96TY08906Rattus norvegicus (Rat)
BH3相互作用域死亡激動劑(Bid)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for BH3 Interacting Domain Death Agonist (Bid)48T/96TY08907Mus musculus (Mouse)
胱天蛋白酶5(CASP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Caspase 5 (CASP5)48T/96TY08908Homo sapiens (Human)
趨化因子C-X-C-基元配體16(CXCL16)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-X-C-Motif Ligand 16 (CXCL16)48T/96TY08909Homo sapiens (Human)
趨化因子C-X-C-基元配體16(CXCL16)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-X-C-Motif Ligand 16 (CXCL16)48T/96TY08910Rattus norvegicus (Rat)
趨化因子C-X-C-基元配體16(CXCL16)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-X-C-Motif Ligand 16 (CXCL16)48T/96TY08911Mus musculus (Mouse)
糖原合酶激酶3α(GSK3α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycogen Synthase Kinase 3 Alpha (GSK3a)48T/96TY08912Homo sapiens (Human)
糖原合酶激酶3α(GSK3α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycogen Synthase Kinase 3 Alpha (GSK3a)48T/96TY08913Rattus norvegicus (Rat)
糖原合酶激酶3α(GSK3α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycogen Synthase Kinase 3 Alpha (GSK3a)48T/96TY08914Mus musculus (Mouse)
B-細(xì)胞淋巴瘤因子2(Bcl2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2)48T/96TY08915Oryctolagus cuniculus (Rabbit)
丙酮酸脫氫酶α(PDHα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pyruvate Dehydrogenase Alpha (PDHa)48T/96TY08916Homo sapiens (Human)
丙酮酸脫氫酶α(PDHα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pyruvate Dehydrogenase Alpha (PDHa)48T/96TY08917Mus musculus (Mouse)
己糖激酶1(HK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hexokinase 1 (HK1)48T/96TY08918Homo sapiens (Human)
己糖激酶1(HK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hexokinase 1 (HK1)48T/96TY08919Mus musculus (Mouse)
己糖激酶1(HK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hexokinase 1 (HK1)48T/96TY08920Rattus norvegicus (Rat)
Ⅳ型膠原(COL4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type IV (COL4)48T/96TY08921Rattus norvegicus (Rat)
中期因子(MK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Midkine (MK)48T/96TY08922Homo sapiens (Human)
胰島素樣生長因子1(IGF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 1 (IGF1)48T/96TY08923Bos taurus; Bovine (Cattle)
同型半胱氨酸(HA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Homocysteic Acid (HA)48T/96TY08924General
細(xì)胞間粘附分子1(ICAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1)48T/96TY08925Canis familiaris; Canine (Dog)
谷胱甘肽S轉(zhuǎn)移酶α1(GSTα1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Alpha 1 (GSTa1)48T/96TY08926Homo sapiens (Human)
谷胱甘肽S轉(zhuǎn)移酶α1(GSTα1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Alpha 1 (GSTa1)48T/96TY08927Rattus norvegicus (Rat)
谷胱甘肽S轉(zhuǎn)移酶α5(GSTα5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Alpha 5 (GSTa5)48T/96TY08928Homo sapiens (Human)
C-型鈉尿肽(CNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C-Type Natriuretic Peptide (CNP)48T/96TY08929Homo sapiens (Human)
氨基己糖苷酶Bβ(HEXβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hexosaminidase B Beta (HEXb)48T/96TY08930Homo sapiens (Human)
氨基己糖苷酶Bβ(HEXβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hexosaminidase B Beta (HEXb)48T/96TY08931Rattus norvegicus (Rat)
氨基己糖苷酶Bβ(HEXβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hexosaminidase B Beta (HEXb)48T/96TY08932Mus musculus (Mouse)
補體因子H(CFH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Factor H (CFH)48T/96T Y08933Homo sapiens (Human)
補體因子H(CFH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Factor H (CFH)48T/96TY08934Rattus norvegicus (Rat)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。