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人抗乙型肝炎病毒表面抗體酶免試劑盒,(HBsAb)ELISA檢測試劑盒 ,英文名:HBsAb ELISA Kit ,英文縮寫:HBsAb ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗乙型肝炎病毒表面抗體ELISA檢測試劑盒,HBsAb 檢測試劑盒, HBsAb 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人抗乙型肝炎病毒表面抗體酶免試劑盒,(HBsAb)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重
激肽釋放酶6(KLK6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kallikrein 6 (KLK6)48T/96TY09185Homo sapiens (Human)
激肽釋放酶8(KLK8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kallikrein 8 (KLK8)48T/96TY09186Homo sapiens (Human)
晚期糖基化終末產(chǎn)物受體(AGER)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Advanced Glycosylation End Product Specific Receptor (AGER)48T/96TY09187Homo sapiens (Human)
ATP結(jié)合盒轉(zhuǎn)運(yùn)蛋白G1(ABCG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATP Binding Cassette Transporter G1 (ABCG1)48T/96TY09188Homo sapiens (Human)
調(diào)節(jié)激活正常T-細(xì)胞表達(dá)分泌因子(RANTES)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)48T/96TY09189Mus musculus (Mouse)
胸腺激活調(diào)節(jié)趨化因子(TARC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thymus Activation Regulated Chemokine (TARC)48T/96TY09190Homo sapiens (Human)
蛋白激酶D1(PRKD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase D1 (PKD1)48T/96TY09191Rattus norvegicus (Rat)
蛋白激酶D1(PRKD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase D1 (PKD1)48T/96TY09192Mus musculus (Mouse)
V-Myc骨髓細(xì)胞瘤病毒癌基因同源物(MYC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)48T/96TY09193Homo sapiens (Human)
V-Fos-FBJ骨肉瘤病毒癌基因同源物(FOS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS)48T/96TY09194Homo sapiens (Human)
V-Jun肉瘤病毒癌基因同源物(JUN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN)48T/96TY09195Homo sapiens (Human)
血小板衍生生長因子B(PDGFB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Derived Growth Factor Subunit B (PDGFB)48T/96TY09196Homo sapiens (Human)
V-Ha-Ras肉瘤病毒癌基因同源物(HRAS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS)48T/96TY09197Homo sapiens (Human)
CD320分子(CD320)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 320 (CD320)48T/96TY09198Rattus norvegicus (Rat)
CD320分子(CD320)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 320 (CD320)48T/96TY09199Mus musculus (Mouse)
基質(zhì)金屬蛋白酶3(MMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 3 (MMP3)48T/96TY09200Oryctolagus cuniculus (Rabbit)
環(huán)加氧酶1(PTGS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostaglandin Endoperoxide Synthase 1 (PTGS1)48T/96TY09201Bos taurus; Bovine (Cattle)
環(huán)加氧酶1(PTGS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostaglandin Endoperoxide Synthase 1 (PTGS1)48T/96TY09202Homo sapiens (Human)
環(huán)加氧酶1(PTGS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostaglandin Endoperoxide Synthase 1 (PTGS1)48T/96TY09203Rattus norvegicus (Rat)
環(huán)加氧酶1(PTGS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostaglandin Endoperoxide Synthase 1 (PTGS1)48T/96TY09204Mus musculus (Mouse)
骨形成蛋白6(BMP6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bone Morphogenetic Protein 6 (BMP6)48T/96TY09205Homo sapiens (Human)
骨形成蛋白6(BMP6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bone Morphogenetic Protein 6 (BMP6)48T/96TY09206Rattus norvegicus (Rat)
骨形成蛋白6(BMP6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bone Morphogenetic Protein 6 (BMP6)48T/96TY09207Mus musculus (Mouse)
親環(huán)素A(CYPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclophilin A (CYPA)48T/96TY09208Mus musculus (Mouse)
血小板因子4(PF4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Factor 4 (PF4)48T/96TY09209Oryctolagus cuniculus (Rabbit)
透明質(zhì)酸結(jié)合蛋白1(HABP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hyaluronan Binding Protein 1 (HABP1)48T/96TY09210Homo sapiens (Human)
透明質(zhì)酸結(jié)合蛋白1(HABP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hyaluronan Binding Protein 1 (HABP1)48T/96TY09211Rattus norvegicus (Rat)
透明質(zhì)酸結(jié)合蛋白1(HABP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hyaluronan Binding Protein 1 (HABP1)48T/96TY09212Mus musculus (Mouse)
鈣結(jié)合蛋白(CR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calretinin (CR)48T/96TY09213Homo sapiens (Human)
鈣結(jié)合蛋白(CR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calretinin (CR)48T/96TY09214Rattus norvegicus (Rat)
鈣結(jié)合蛋白(CR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calretinin (CR)48T/96TY09215Mus musculus (Mouse)
155kDa核孔蛋白(NUP155)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleoporin 155kDa (NUP155)48T/96TY09216Homo sapiens (Human)
腎上腺素能受體α1A(ADRα1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adrenergic Receptor Alpha 1A (ADRa1A)48T/96TY09217Homo sapiens (Human)
多巴胺受體D1(DRD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dopamine Receptor D1 (DRD1)48T/96TY09218Homo sapiens (Human)
CD28分子(CD28)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 28 (CD28)48T/96TY09219Homo sapiens (Human)
不對稱二甲基精氨酸(ADMA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Asymmetrical Dimethylarginine (ADMA)48T/96TY09220General
野鼠色基因相關(guān)蛋白(AGRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Agouti Related Protein (AGRP)48T/96TY09221Homo sapiens (Human)
集聚蛋白(AGRN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Agrin (AGRN)48T/96TY09222Homo sapiens (Human)
補(bǔ)體C5轉(zhuǎn)化酶(C5c)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement C5 Convertase (C5c)48T/96TY09223Homo sapiens (Human)
補(bǔ)體成分4b(C4b)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 4b (C4b)48T/96TY09224Homo sapiens (Human)
補(bǔ)體成分8β(C8β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 8b (C8b)48T/96TY09225Homo sapiens (Human)
多效生長因子(PTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pleiotrophin (PTN)48T/96TY09226Homo sapiens (Human)
彈性蛋白酶4(ELA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Elastase 4 (ELA4)48T/96TY09227Homo sapiens (Human)
阿黑皮素原(POMC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proopiomelanocortin (POMC)48T/96TY09228Homo sapiens (Human)
分泌性白細(xì)胞蛋白酶抑制因子(SLPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI)48T/96TY09229Homo sapiens (Human)
谷胱甘肽還原酶(GR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione Reductase (GR)48T/96TY09230Homo sapiens (Human)
前膠原C端蛋白酶增強(qiáng)子1(PCPE1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procollagen C Proteinase Enhancer 1 (PCPE1)48T/96TY09231Homo sapiens (Human)
穿孔素1(PRF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Perforin 1 (PRF1)48T/96T 人抗乙型肝炎病毒表面抗體酶免試劑盒,(HBsAb)ELISA檢測試劑盒 Y09232Homo sapiens (Human)
cAMP應(yīng)答元件結(jié)合蛋白(CREB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclic AMP Response Element Binding Protein (CREB)48T/96TY09233Homo sapiens (Human)
微管關(guān)聯(lián)蛋白4(MAP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Microtubule Associated Protein 4 (MAP4)48T/96TY09234Homo sapiens (Human)
各種類型ELISA測定時,人抗乙型肝炎病毒表面抗體酶免試劑盒,(HBsAb)ELISA檢測試劑盒 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。