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人抗斑疹傷寒抗體酶免試劑盒,(anti-typhus-Ab)ELISA檢測(cè)試劑盒 ,英文名:anti-typhus-Ab ELISA Kit ,英文縮寫:anti-typhus-Ab ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗斑疹傷寒抗體ELISA檢測(cè)試劑盒,anti-typhus-Ab 檢測(cè)試劑盒, anti-typhus-Ab 檢測(cè)試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。人抗斑疹傷寒抗體酶免試劑盒,(anti-typhus-Ab)ELISA檢測(cè)試劑盒 在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測(cè)定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測(cè)中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競(jìng)爭(zhēng)法及定量檢測(cè)影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過(guò)程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無(wú)關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過(guò)程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測(cè)讀儀(酶標(biāo)儀),比色法判讀可選擇單波長(zhǎng)或雙波長(zhǎng),根據(jù)反應(yīng)液顏色的不同選用不同波長(zhǎng)的濾光片,以空白孔校零測(cè)定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
Endoglin蛋白(ENG)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Endoglin (ENG)48T/96TY09635Mus musculus (Mouse)
細(xì)胞絨毛蛋白(CVL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cytovillin (CVL)48T/96TY09636Homo sapiens (Human)
通用轉(zhuǎn)錄因子ⅡE肽1(GTF2E1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for General Transcription Factor IIE, Polypeptide 1 (GTF2E1)48T/96TY09637Homo sapiens (Human)
殺傷細(xì)胞凝集素樣受體亞家族C成員2(KLRC2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Killer Cell Lectin Like Receptor Subfamily C, Member 2 (KLRC2)48T/96TY09638Homo sapiens (Human)
煙堿型膽堿受體β2(CHRNβ2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2)48T/96TY09639Homo sapiens (Human)
白介素1受體關(guān)聯(lián)激酶1(IRAK1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 1 Receptor Associated Kinase 1 (IRAK1)48T/96TY09640Homo sapiens (Human)
白介素1受體關(guān)聯(lián)激酶2(IRAK2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 1 Receptor Associated Kinase 2 (IRAK2)48T/96TY09641Homo sapiens (Human)
顆粒溶素(GNLY)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Granulysin (GNLY)48T/96TY09642Homo sapiens (Human)
白介素1受體關(guān)聯(lián)激酶4(IRAK4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 1 Receptor Associated Kinase 4 (IRAK4)48T/96TY09643Homo sapiens (Human)
白介素1受體關(guān)聯(lián)激酶3(IRAK3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 1 Receptor Associated Kinase 3 (IRAK3)48T/96TY09644Homo sapiens (Human)
黏膜地址素細(xì)胞黏附分子(MAdCAM1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1)48T/96TY09645Homo sapiens (Human)
肺部激活調(diào)節(jié)趨化因子(PARC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Pulmonary Activation Regulated Chemokine (PARC)48T/96TY09646Homo sapiens (Human)
維甲酸誘導(dǎo)基因1蛋白(RIG1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Retinoic Acid Inducible Gene 1 Protein (RIG1)48T/96TY09647Homo sapiens (Human)
趨化因子C-C-基元受體1(CCR1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Chemokine C-C-Motif Receptor 1 (CCR1)48T/96TY09648Homo sapiens (Human)
絲氨酸蛋白酶2(PRSS2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Protease, Serine 2 (PRSS2)48T/96TY09649Homo sapiens (Human)
表面活性物質(zhì)關(guān)聯(lián)蛋白A(SPA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Surfactant Associated Protein A (SPA)48T/96TY09650Canis familiaris; Canine (Dog)
血清淀粉樣P物質(zhì)(SAP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Serum Amyloid P Component (SAP)48T/96TY09651Homo sapiens (Human)
抗糖脂抗體(AGA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Anti-Glycolipid Antibody (AGA)48T/96TY09652Homo sapiens (Human)
甘露糖受體C1(MRC1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Mannose Receptor C Type 1 (MRC1)48T/96TY09653Homo sapiens (Human)
甘露糖(MN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Mannose (MN)48T/96TY09654General
脂阿拉伯甘露聚糖(LAM)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Lipoarabinomannan (LAM)48T/96TY09655Homo sapiens (Human)
吲哚胺2,3-雙加氧酶(IDO)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Indoleamine-2,3-Dioxygenase (IDO)48T/96TY09656Homo sapiens (Human)
肽基脯氨酰異構(gòu)酶F(PPIF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Peptidylprolyl Isomerase F (PPIF)48T/96TY09657Homo sapiens (Human)
Z-DNA結(jié)合蛋白1(ZBP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Z-DNA Binding Protein 1 (ZBP1)48T/96TY09658Homo sapiens (Human)
八聚體結(jié)合轉(zhuǎn)錄因子1(OCT1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Octamer Binding Transcription Factor 1 (OCT1)48T/96TY09659Homo sapiens (Human)
八聚體結(jié)合轉(zhuǎn)錄因子2(OCT2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Octamer Binding Transcription Factor 2 (OCT2)48T/96TY09660Homo sapiens (Human)
大型多功能肽酶7(LMP7)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Large Multifunctional Peptidase 7 (LMP7)48T/96TY09661Homo sapiens (Human)
細(xì)胞色素P450家族成員21B(CYP21B)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cytochrome P450 21B (CYP21B)48T/96TY09662Homo sapiens (Human)
絲切蛋白1(CFL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cofilin 1, Non Muscle (CFL1)48T/96TY09663Homo sapiens (Human)
淋巴毒素β(LTβ)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Lymphotoxin Beta (LTb)48T/96TY09664Homo sapiens (Human)
干擾素調(diào)節(jié)因子1(IRF1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interferon Regulatory Factor 1 (IRF1)48T/96TY09665Homo sapiens (Human)
糖基化依賴性細(xì)胞黏附分子(GlyCAM1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glycosylation Dependent Cell Adhesion Molecule 1 (GlyCAM1)48T/96TY09666Homo sapiens (Human)
血小板因子4V1(PF4V1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Plaet Factor 4 Variant 1 (PF4V1)48T/96TY09667Homo sapiens (Human)
干擾素ω(IFNω)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interferon Omega (IFNw)48T/96TY09668Homo sapiens (Human)
粒細(xì)胞趨化蛋白2(GCP2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Granulocyte Chemotactic Protein 2 (GCP2)48T/96TY09669Homo sapiens (Human)
IgG-Fc片段受體Ⅰ(FcγRⅠ)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Receptor I For The Fc Region Of Immunoglobulin G (FcgRI)48T/96TY09670Homo sapiens (Human)
IgG-Fc片段受體Ⅱ(FcγRⅡ)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Receptor II For The Fc Region Of Immunoglobulin G (FcgRII)48T/96TY09671Homo sapiens (Human)
細(xì)胞附著蛋白1(CYTH1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cytohesin 1 (CYTH1)48T/96TY09672Homo sapiens (Human)
IgE-Fc受體Ⅰ(FcεRⅠ)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Receptor I For The Fc Region Of Immunoglobulin E (FceRI)48T/96TY09673Homo sapiens (Human)
IgE-Fc片段受體Ⅱ(FcεRⅡ)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Receptor II For The Fc Region Of Immunoglobulin E (FceRII)48T/96TY09674Homo sapiens (Human)
細(xì)胞附著蛋白2(CYTH2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cytohesin 2 (CYTH2)48T/96TY09675Homo sapiens (Human)
單核細(xì)胞巨噬細(xì)胞分化關(guān)聯(lián)蛋白(MMA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Monocyte To Macrophage Differentiation Associated Protein (MMA)48T/96TY09676Homo sapiens (Human)
磷脂酰肌醇蛋白聚糖2(GPC2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glypican 2 (GPC2)48T/96TY09677Homo sapiens (Human)
整合素β2(ITGβ2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Integrin Beta 2 (ITGb2)48T/96TY09678Homo sapiens (Human)
整合素連鎖激酶(ILK)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Integrin Linked Kinase (ILK)48T/96TY09679Homo sapiens (Human)
信號(hào)轉(zhuǎn)導(dǎo)銜接分子1(STAM1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Signal Transducing Adaptor Molecule 1 (STAM1)48T/96TY09680Homo sapiens (Human)
干擾素調(diào)節(jié)因子3(IRF3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interferon Regulatory Factor 3 (IRF3)48T/96TY09681Homo sapiens (Human)
含鈀蛋白(PALLD)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Palladin (PALLD)48T/96TY09682Homo sapiens (Human)
胱天蛋白酶1(CASP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) ELISA Kit for Caspase 1 (CASP1)48T/96TY09683Homo sapiens (Human)
酪氨酸激酶2(Tyk2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tyrosine Kinase 2 (Tyk2)48T/96TY09684Homo sapiens (Human)
各種類型ELISA測(cè)定時(shí), 一般需經(jīng)過(guò)以下步驟:固相包被→加待測(cè)樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。