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小鼠17羥皮質(zhì)類固醇酶免試劑盒,(17-OHCS)ELISA檢測(cè)試劑盒,英文名:17-OHCS ELISA Kit,英文縮寫:17-OHCS ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠17羥皮質(zhì)類固醇ELISA檢測(cè)試劑盒,17-OHCS 檢測(cè)試劑盒,17-OHCS 檢測(cè)試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠17羥皮質(zhì)類固醇酶免試劑盒,(17-OHCS)ELISA檢測(cè)試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測(cè)定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測(cè)中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競(jìng)爭(zhēng)法及定量檢測(cè)影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測(cè)讀儀(酶標(biāo)儀),比色法判讀可選擇單波長(zhǎng)或雙波長(zhǎng),根據(jù)反應(yīng)液顏色的不同選用不同波長(zhǎng)的濾光片,以空白孔校零測(cè)定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
堿性鞘磷脂磷酸二酯酶(Alk-Smase)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Alkaline Sphingomyelinase (Alk-Smase)48T/96TY10955Mus musculus (Mouse)
堿性鞘磷脂磷酸二酯酶(Alk-Smase)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Alkaline Sphingomyelinase (Alk-Smase)48T/96TY10956Homo sapiens (Human)
膽囊收縮素(CCK)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cholecystokinin (CCK)48T/96TY10957Rattus norvegicus (Rat)
膽囊收縮素(CCK)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cholecystokinin (CCK)48T/96TY10958Mus musculus (Mouse)
膽囊收縮素(CCK)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cholecystokinin (CCK)48T/96TY10959Homo sapiens (Human)
胰高血糖素樣肽1(GLP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glucagon Like Peptide 1 (GLP1)48T/96TY10960Rattus norvegicus (Rat)
胰高血糖素樣肽1(GLP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glucagon Like Peptide 1 (GLP1)48T/96TY10961Mus musculus (Mouse)
胰高血糖素樣肽1(GLP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glucagon Like Peptide 1 (GLP1)48T/96TY10962Homo sapiens (Human)
骨成型蛋白7(BMP7)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Bone Morphogenetic Protein 7 (BMP7)48T/96TY10963Homo sapiens (Human)
骨成型蛋白7(BMP7)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Bone Morphogenetic Protein 7 (BMP7)48T/96TY10964Rattus norvegicus (Rat)
骨成型蛋白7(BMP7)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Bone Morphogenetic Protein 7 (BMP7)48T/96TY10965Mus musculus (Mouse)
淋巴細(xì)胞功能關(guān)聯(lián)抗原3(LFA3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Lymphocyte Function Associated Antigen 3 (LFA3)48T/96TY10966Homo sapiens (Human)
淋巴細(xì)胞功能關(guān)聯(lián)抗原2(LFA2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Lymphocyte Function Associated Antigen 2 (LFA2)48T/96TY10967Rattus norvegicus (Rat)
淋巴細(xì)胞功能關(guān)聯(lián)抗原2(LFA2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Lymphocyte Function Associated Antigen 2 (LFA2)48T/96TY10968Mus musculus (Mouse)
血紅蛋白μ(HBμ)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Hemoglobin Mu (HBm)48T/96TY10969Homo sapiens (Human)
胰島素受體(ISR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Receptor (ISR)48T/96TY10970Homo sapiens (Human)
胰島素受體(ISR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Receptor (ISR)48T/96TY10971Mus musculus (Mouse)
胰島素受體(ISR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Receptor (ISR)48T/96TY10972Rattus norvegicus (Rat)
促腎上皮質(zhì)激素釋放激素(CRH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Corticotropin Releasing Hormone (CRH)48T/96TY10973Mus musculus (Mouse)
促甲狀腺素釋放激素(TRH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Thyrotropin Releasing Hormone (TRH)48T/96TY10974Homo sapiens (Human)
促甲狀腺素釋放激素(TRH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Thyrotropin Releasing Hormone (TRH)48T/96TY10975Mus musculus (Mouse)
誘導(dǎo)型一氧化氮合酶(NOS2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2)48T/96TY10976Homo sapiens (Human)
抑制素βA(INHβA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Inhibin Beta A (INHbA)48T/96TY10977Homo sapiens (Human)
抑制素βA(INHβA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Inhibin Beta A (INHbA)48T/96TY10978Rattus norvegicus (Rat)
抑制素βA(INHβA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Inhibin Beta A (INHbA)48T/96TY10979Mus musculus (Mouse)
促性腺激素釋放激素(GnRH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Gonadotropin Releasing Hormone (GnRH)48T/96TY10980Mus musculus (Mouse)
促微管蛋白聚合蛋白(TPPP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tubulin Polymerization Promoting Protein (TPPP)48T/96TY10981Homo sapiens (Human)
促微管蛋白聚合蛋白(TPPP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tubulin Polymerization Promoting Protein (TPPP)48T/96TY10982
促微管蛋白聚合蛋白(TPPP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tubulin Polymerization Promoting Protein (TPPP)48T/96TY10983Mus musculus (Mouse)
核因子κB受體激活因子配基(RANκL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)48T/96TY10984Homo sapiens (Human)
核因子κB受體激活因子配基(RANκL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)48T/96TY10985Rattus norvegicus (Rat)
核因子κB受體激活因子配基(RANκL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)48T/96TY10986Mus musculus (Mouse)
凝溶膠蛋白(GS)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Gelsolin (GS)48T/96TY10987Homo sapiens (Human)
凝溶膠蛋白(GS)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Gelsolin (GS)48T/96TY10988Rattus norvegicus (Rat)
凝溶膠蛋白(GS)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Gelsolin (GS)48T/96TY10989Mus musculus (Mouse)
血管生成素樣蛋白1(ANGPTL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Angiopoietin Like Protein 1 (ANGPTL1)48T/96TY10990Homo sapiens (Human)
血管生成素樣蛋白1(ANGPTL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Angiopoietin Like Protein 1 (ANGPTL1)48T/96TY10991Rattus norvegicus (Rat)
血管生成素樣蛋白1(ANGPTL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Angiopoietin Like Protein 1 (ANGPTL1)48T/96TY10992Mus musculus (Mouse)
上皮中性粒細(xì)胞激活肽78(ENA78)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78)48T/96TY10993Homo sapiens (Human)
上皮中性粒細(xì)胞激活肽78(ENA78)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78)48T/96TY10994Rattus norvegicus (Rat)
上皮中性粒細(xì)胞激活肽78(ENA78)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78)48T/96TY10995Mus musculus (Mouse)
10kDa干擾素γ誘導(dǎo)蛋白(IP10)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10)48T/96TY10996Homo sapiens (Human)
10kDa干擾素γ誘導(dǎo)蛋白(IP10)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10)48T/96TY10997Rattus norvegicus (Rat)
10kDa干擾素γ誘導(dǎo)蛋白(IP10)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10)48T/96TY10998Mus musculus (Mouse)
GATA結(jié)合蛋白4(GATA4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for GATA Binding Protein 4 (GATA4)48T/96TY10999Homo sapiens (Human)
GATA結(jié)合蛋白4(GATA4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for GATA Binding Protein 4 (GATA4)48T/96TY11000Rattus norvegicus (Rat)
GATA結(jié)合蛋白4(GATA4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for GATA Binding Protein 4 (GATA4)48T/96TY11001Mus musculus (Mouse)
結(jié)蛋白(Des)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Desmin (Des)48T/96TY11002Homo sapiens (Human)
結(jié)蛋白(Des)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Desmin (Des)48T/96TY11003Rattus norvegicus (Rat)
結(jié)蛋白(Des)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Desmin (Des)48T/96TY11004Mus musculus (Mouse)
肌球蛋白ⅠA(MYO1A)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Myosin IA (MYO1A)48T/96TY11005Homo sapiens (Human)
肌球蛋白ⅠA(MYO1A)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Myosin IA (MYO1A)48T/96TY11006Rattus norvegicus (Rat)
肌球蛋白ⅠA(MYO1A)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Myosin IA (MYO1A)48T/96TY11007Mus musculus (Mouse)
輔肌動(dòng)蛋白α2(ACTN2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Actinin Alpha 2 (ACTN2)48T/96TY11008Homo sapiens (Human)
垂體腺苷酸環(huán)化酶激活肽(ADCYAP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1)48T/96TY11009Mus musculus (Mouse)
表皮生長(zhǎng)因子受體2(EGFR2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Epidermal Growth Factor Receptor 2 (EGFR2)48T/96TY11010Mus musculus (Mouse)
松弛肽(RLN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Relaxin (RLN)48T/96TY11011Sus scrofa; Porcine (Pig)
甘露聚糖結(jié)合凝集素關(guān)聯(lián)絲氨酸蛋白酶1(MASP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Mannose Associated Serine Protease 1 (MASP1)48T/96TY11012Homo sapiens (Human)
凝集素(PHA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Phytohemagglutinin (PHA)48T/96TY11013Spinacia oleracea (Spinach)
蛙皮素6-14(BB)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Bombesin 6-14 (BB)48T/96TY11014General
粘蛋白5B(MUC5B)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Mucin 5 Subtype B (MUC5B)48T/96TY11015Mus musculus (Mouse)
胰高血糖素(GC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glucagon (GC)48T/96TY11016Chicken (Gallus)
白介素1β(IL1β)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 1 Beta (IL1b)48T/96TY11017Cavia (Guinea pig )
白介素8(IL8)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 8 (IL8)48T/96TY11018Cavia (Guinea pig )
白介素10(IL10)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 10 (IL10)48T/96TY11019Cavia (Guinea pig )
白介素33(IL33)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 33 (IL33)48T/96TY11020Mus musculus (Mouse)
α2-HS糖蛋白(αHSG)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG)48T/96TY11021Sus scrofa; Porcine (Pig)
纖維蛋白原α(FGα)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibrinogen Alpha (FGa)48T/96TY11022Mus musculus (Mouse)
纖維蛋白原α(FGα)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibrinogen Alpha (FGa)48T/96TY11023Rattus norvegicus (Rat)
纖維蛋白原α(FGα)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibrinogen Alpha (FGa)48T/96TY11024Oryctolagus cuniculus (Rabbit)
接觸蛋白3(CNTN3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Contactin 3 (CNTN3)48T/96TY11025Rattus norvegicus (Rat)
細(xì)胞胱硫醚γ裂解酶(CSE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cystathionine Gamma Lyase (CSE)48T/96TY11026Homo sapiens (Human)
胰島素降解酶(IDE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Degrading Enzyme (IDE)48T/96TY11027Mus musculus (Mouse)
氧鯊烯環(huán)化酶(OSC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Oxidosqualene Cyclase (OSC)48T/96TY11028Rattus norvegicus (Rat)
氧鯊烯環(huán)化酶(OSC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Oxidosqualene Cyclase (OSC)48T/96TY11029Homo sapiens (Human)
α-乳清蛋白(αLA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Alpha-Lactalbumin (aLA)48T/96TY11030Bos taurus; Bovine (Cattle)
泛素(Ub)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Ubiquitin (Ub)48T/96TY11031Rattus norvegicus (Rat)
白介素17(IL17)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 17 (IL17)48T/96TY11032Canis familiaris; Canine (Dog)
垂體腺苷酸環(huán)化酶激活肽(ADCYAP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1)48T/96TY11033Rattus norvegicus (Rat)
前胰淀粉樣蛋白(ProIAPP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Proislet Amyloid Polypeptide (ProIAPP)48T/96TY11034Homo sapiens (Human)
各種類型ELISA測(cè)定時(shí),一般需經(jīng)過以下步驟:固相包被→加待測(cè)樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。